Strauss-Kahn was accused of assaulting an employee cleaning a hotel in Manhattan, New York, where he was staying on 14 May.
The allegations that led to him being arrested when it was in the plane would take off to Paris.
The official statement in court that he was not guilty of this signifies a court case against him would be long running.
Court proceedings against him is expected to begin some months away.
The police catch him with seven charges on May 15, including rape, a felony sexual assault, menyekap someone unlawfully and touch with coercion.
Strauss-Kahn languishing in custody for four days in prison Island hide drugs, before being released on bail.
If convicted, Strauss-Kahn faces a maximum penalty of 25 years in prison.
Strauss-Kahn arrived at the courthouse with his wife, television journalist France Anne Sinclair.
A group of hotel workers that are outside the courthouse shouted, ' you are embarrassing! ' as the action of solidarity with the employees of the hotel who accused Strauss-Kahn attacked him.